Figure 2

Conformation of the GatD/MurT complex in solution. (a) GatD/MurT crystal structure before (top) and after (bottom) refinement against SAXS data. While the overall organization and shape of the complex remain similar, the increased diameter of the GatD and MurT middle domain envelopes as well as the smaller gap of 22 Å instead of 32 Å between them suggest that some flexibility between domains exists in solution. (b) Fit of synthetic scattering profiles derived from the crystal structure (top) and refined model (bottom) against the experimental SAXS data.