Figure 5
CAFs enhance invasiveness of patient-derived PDAC cells. The 3D tumor-tissue invasion model was prepared with 200 Pa Oligomer for both the tumor and surrounding tissue compartments. Tumor compartments were created with 10.05 alone or 10.05 + CAFS (at 1:1 ratio) at 1 × 107 cells/mL in Oligomer and cultured 4 days. (a) Images represent nine fields of view, each of which is a maximum projection of a 400 μm confocal z-stack. Red = tumor cells (TdT) and green = CAFs (EGFP). Scale bars = 200 μm. (b) Images represent maximum projections of 10 μm confocal z-stacks from cryosectioned constructs. Confocal reflection microscopy (white) was used to visualize matrix microstructure. Yellow arrowheads denote matrix alignment and remodeling; scale bars = 20 μm. (c) Images represent maximum projections of a 20 μm confocal z-stacks of cryosectioned constructs stained for E-cadherin (yellow) and vimentin (blue). Final panel represents a 3X zoom of boxed region in overlay panel. White arrowhead denotes direct interaction between tumor cell and CAF; scale bars = 50 μm.
