Figure 6

Proof-of-concept drug screen demonstrates HT-HC screening capacity. The 3D tumor-tissue invasion model was prepared with 200 Pa Oligomer for both the tumor and surrounding tissue compartments. Tumor compartments were prepared with 1 × 10⁷ Panc-1 or BxPC-3 cells/mL in Oligomer and treated with serial dilutions of gemcitabine, 20 μM STS (cytotoxicity control), and 1% DMSO (vehicle control) for 4 days. (a) Images were obtained using an Opera Phenix. Representative images from a Panc-1 experiment represent maximum projections of 500 μm z-stacks. Columns represent different wells of a 96-well plate; rows represent (i) overlay of all channels, (ii) Click-it EdU 488 stained proliferating cells, (iii) MitoTracker Red stained active mitochondria, and (iv) Hoechst 33342 stained nuclei. (b) Representative dose response curves for (i) proliferative capacity, (ii) metabolic activity, and (iii) invasion for Panc-1 and BxPC-3. (c) Calculated (i) IC₅₀ and (ii) E_max values obtained from independent experiments (N = 3) and compared with Tukey-adjusted multiple comparisons. Letters over bars (mean ± SD) denote statistical differences; specifically, bars with the same letter are not statistically different, while those with different letters are statistically different (p < 0.05).