Figure 1
From: Septins are critical regulators of osteoclastic bone resorption
The SEPT9 gene is expressed during human OC differentiation and different isoforms of SEPT9 protein can be detected. (A) Q-RT-PCR of SEPT9, n = 3 biological replicates, representative of three independent experiments with similar results. Statistics: Kruskal-Wallis test, two-tailed, **p = 0.0015; Dunn’s multiple comparisons test, **p = 0.0076. (B) Q-RT-PCR of CATK, n = 3 biological replicates (same as in A), representative of three independent experiments with similar results. Statistics: Kruskal-Wallis test, two-tailed, ***p < 0.0001; Dunn’s multiple comparisons test, **p = 0.0041. (C) Western blot of SEPT9 protein representative of three independent experiments with similar results. (D) Western blot of CatK protein (same cell lysates as in C representative of three independent experiments with similar results. §, pro-CatK; *, active CatK. C and D show cropped Western blot images; full size uncropped unedited blots can be seen in Supplementary Figure S1.
