Figure 3
From: Septins are critical regulators of osteoclastic bone resorption
Confocal IF detection of SEPT9 shows that it is primarily allocated to the sites of the human OC engaged in active bone resorption and to those located within the boundaries of the trench. (A–C) 3D z-projection of trench resorbing OC. Scale bar: 20 µm. Please refer to Supplementary Video S1 for a rotating version of Fig. 3C. (D) Cross-section (xz) through the length axis of the OC and trench as indicated by white dashed lines in A to C. Scale bars: 20 µm. E-H) A single confocal plane (0.55 µm) (xy) at the trench level within the cavity. Scale bars: 20 µm. (I–L) A single confocal plane (0.55 µm) (xy) at the bone surface level. (M–O) These images show the enlarged insets indicated by a white box in I to K. Scale bars: 20 µm. (P) Left: Shows an enlargement of the areas indicated by a white box in M to O. Scale bar: 2 µm; arrows point to fibres positive for both F-actin and SEPT9. Right: Line scan through area marked by yellow dashed line in the lower left image. Colors: green, phalloidin/F-actin; red, IF staining of SEPT9; blue, dapi staining; grey, phase contrast; yellow, co-localization of phalloidin and SEPT9. Representative of 15 experiments.
