Figure 5
From: Septins are critical regulators of osteoclastic bone resorption
Pit-making human OC: Confocal IF detection of SEPT9 and clathrin. (A) A single confocal plane (xy) at the level of the sealing zone. Scale bar: 20 µm. (B–D) A single confocal plane (0.55 µm) (xy) showing an enlargement of the area indicated by a white box in A. Scale bars: 5 µm. White arrow heads point to F-actin and SEPT9 fibers that seem to be attached to one another. (E–H) A single confocal plane (xy) a few planes deeper than the one shown in A, at the transition between the bone surface and the beginning of the cavity. Scale bars: 20 µm. The white solid line marks the inside of the sealing zone while the yellow dashed line indicates the perimeter of the cavity. (I–P) Show different representations of the same confocal plane (xy) within the pit-cavity. Scale bars: 10 µm. (Q) Cross-section (xz) through the OC and pit as indicated by a white line in L. Scale bar: 10 µm. (R) Line scan through the area marked by a white line in D. (S) Line scan through the resorptive zone as indicated by a yellow dashed line in Q. Colors: green, phalloidin/F-actin; red, IF staining of SEPT9; blue, IF staining of clathrin; grey, phase contrast; purple, co-localization of SEPT9 and clathrin. Representative of 5 experiments.
