Figure 6
From: Septins are critical regulators of osteoclastic bone resorption
SEPT9-containing fibrils strongly co-localize with the microtubular network in human bone resorbing OCs. (A and C) 3D z-projection of trench-making OC. Scale bars: 20 µm. (B) Phase contrast image of trench cavity made by the OC shown in A and C. Yellow dashed line indicates the perimeter of the trench. Scale bar: 20 µm. (D–H) Zoom in on the highlighted zone in A. Shown is one confocal z-plane (0.55 µm) with the indicated staining. Scale bars: 5 µm. (I–K) Zoom in on the highlighted zone in H. Shown is one confocal z-plane with the indicated staining. White arrows point to examples of joined microtubule and SEPT9 containing filaments. Scale bars: 2.5 µm. (L) Profile view through the OC along the axis indicated by a white dotted line in A. (M–O) Show selected z-planes through OC as indicated by the orange arrows in L, representing the top middle and bottom fractions of the OC, respectively. Scale bars: 10 µm. Colors: green, phalloidin/F-actin; red, IF staining of SEPT9; blue, IF staining of α-tubulin; grey, phase contrast; purple, co-localization of SEPT9 and α-tubulin. Representative of 5 experiments.
