Figure 2

PTPN22 does not regulate immune complex induced maturation of BMDCs. (a,b) WT or Ptpn22^−/− BMDCs were stimulated in the presence of ova, anti-ova or ova IC. After 24 hours, BMDCs were harvested and surface stained for CD80, CD86 and MHCII. (a) Representative flow cytometry plots showing cell surface expression of CD80, CD86 and MHCII on WT (top) and Ptpn22^−/− (bottom) BMDCs after 24 hour stimulation with ova (red), anti-ova (blue), ova IC (black), or left unstimulated (grey). (b) Pooled data (geometric mean fluorescence intensity, GMFI, gated on live, CD11c⁺ singlets) are shown for WT (white) and Ptpn22^−/− (black) BMDC CD80 (left), CD86 (middle) and MHCII (right) expression. n = 6–8 + s.d.; ***p < 0.005, **p < 0.01 using a 2-way ANOVA with Tukey’s multiple comparisons test. All WT v Ptpn22^−/− comparisons were non-significant (n.s.). Bars on graphs only show comparisons of ova IC v unstim/ova/anti-ova for Ptpn22^−/− BMDCs (for simplicity).