Figure 2 | Scientific Reports

Figure 2

From: Electrophilic nitroalkene-tocopherol derivatives: synthesis, physicochemical characterization and evaluation of anti-inflammatory signaling responses

Figure 2

Reaction of synthetic nitroalkenes with nucleophiles in different environments. (A) Spectral change upon reaction of 10 µM NATOH with 0.1 mM βME in TMA20 buffer at pH 8.1 containing 2.5 g/L SDS. (B) First-order plots obtained at different concentrations of βME measuring absorbance at 350 nm. (C) Second-order plot for the same experiment, linear fit parameters kf (slope) = 2.3 M−1 s−1, kr (intercept) = 5 × 10−3 s−1. (D) Spectral change upon titration of NATOH in CHAPS micelles. The arrows indicate the change upon increase in pH of the bulk solution. (E) Titration curves of NATOH at 350 nm in TMA20 buffer and different micelle suspensions, from left to right, CTAB, CHAPS, Triton X-100 and SDS. The absorbance was normalized to the acidic and alkaline extrapolated values of the fit. (F) Apparent pKa results of NATOH and NATxME in different micellar media.

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