Figure 3 | Scientific Reports

Figure 3

From: Electrophilic nitroalkene-tocopherol derivatives: synthesis, physicochemical characterization and evaluation of anti-inflammatory signaling responses

Figure 3

NATxME anti-inflammatory and cytoprotective effects. NATxME inhibits the secretion of pro-inflammatory cytokines (A–C). RAW 264.7 macrophages were treated with NATxME 1, 3 and 10 µM for 2 h and then stimulated with LPS (50 ng/ml) for 18 h. The secretion of inflammatory cytokines MCP-1 (A), IL-6 (B) and TNF-α (C) was analyzed by ELISA in the supernatant. Values are shown as mean ± SD. Statistical analysis: one-way ANOVA Bonferroni’s multiple comparison test. (A) * = 0.0294; ** = 0.0017; **** < 0.0001. (B) ** = 0.013; **** < 0.0001. (C) * = 0.0131. vs. LPS. NATxME induces the Nrf2-Keap-1 system (D–F). Raw 264.7 macrophages were treated with NATxME 1, 3 and 10 µM for 5 h. The expression of NQO1 (D), GCLM (E) and HO-1 (F) were analyzed by quantitative RT-PCR. Values are shown as mean ± SD. Statistical analysis: one-way ANOVA Bonferroni’s multiple comparison test. (D) ** = 0.0038. (E) * = 0.0310; *** = 0.0001. (F) *** = 0.0001. vs control.

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