Figure 4
Mechanical properties of the C. glutamicum and E. coli membrane. (A) Representative images of how the C. glutamicum (top) and E. coli (bottom) membrane expand in response to the application of negative pressure (−10 mmHg steps), respectively. (B) The quasi-linear relationship between the membrane tension and the areal strain is shown for C. glutamicum (left) and E. coli (right). The slopes in these diagrams represent the areal elasticity modulus KA for each membrane. (C) The comparison of KA between the C. glutamicum and E. coli membrane. The experiments were performed more than three times independently. Bars show standard error (**student’s t-test, P < 0.01). (D) The solvated C. glutamicum lipid bilayer composed of their lipid components. POPG (yellow), POPI (blue), and cardiolipin (green) are shown, and the cross-sectional areas of the lipid along the bilayer thickness are shown as Ai (i = 1, 2, …, N) (left). The E. coli lipid bilayer composed of their lipid components (right). Four types of PE lipids (POPE, PMPE, QMPE, and OSPE) and two types of PG (PMPG and PSPG) are shown (See Supplemental Table S1). (E) The relationship between membrane tension and areal strain is shown for the C. glutamicum and E. coli lipid bilayers. (F) The comparison of KA estimated by MD simulations between the C. glutamicum and E. coli membrane lipid bilayers. Three data points of independent simulations were analyzed, and bars represent SEM (**student’s t-test, P < 0.01).
