Figure 5
Reconstructed volume in x-y and y-z planes from liver sample cut at increments of 25 nm and imaged with primary energies of 1.0 keV and 1.4 keV. (a) Low-magnification BSE image of hepatocyte in the x-y plane acquired at primary energy of 1.4 keV. (b) Region indicated by square in (a) at higher magnification showing membranes of endoplasmic reticulum. (c) Average of 1.0 keV and 1.4 keV BSE image intensities displayed in the y-z plane. (d) Calculated sub-slice stack in the y-z plane showing improved spatial resolution relative to (c), but with additional noise. (e) Five-slice average in the x-direction of y-z view in (c). (f) Five-slice average in the x-direction of y-z view in (d). Arrows in (c–f) denote areas with improved resolution. Scale bar in (a), 5 µm; and in (b–f), 500 nm.
