Table 2 Quality control of reads preprocessing.
From: Sexual difference of small RNA expression in Tetralogy of Fallot
Sample | Total reads | N% > 10% | Low quality | 5′adapter contamination | 3′adapter null or insert null | With polly A/T/G/C | Clean reads |
|---|---|---|---|---|---|---|---|
tof1 | 11592168 (100.00%) | 2632 (0.02%) | 66848 (0.58%) | 157 (0.00%) | 99645 (0.86%) | 4770 (0.04%) | 11418116 (95.80%) |
tof2 | 10268410 (100.00%) | 2265 (0.02%) | 58783 (0.57%) | 404 (0.00%) | 96020 (0.94%) | 6406 (0.06%) | 10104532 (98.40%) |
tof3 | 13857585 (100.00%) | 1737 (0.01%) | 108448 (0.78%) | 206 (0.00%) | 107758 (0.78%) | 6225 (0.04%) | 13633211 (98.38%) |
tof4 | 12425771 (100.00%) | 1468 (0.01%) | 89623 (0.72%) | 289 (0.00%) | 111450 (0.90%) | 5102 (0.04%) | 12217839 (98.33%) |
tof6 | 10044411 (100.00%) | 1202 (0.01%) | 44532 (0.44%) | 292 (0.00%) | 113812 (1.13%) | 9080 (0.09%) | 9875493 (98.32%) |
tof7 | 10652214 (100.00%) | 744 (0.01%) | 33063 (0.31%) | 117 (0.00%) | 120102 (1.13%) | 4662 (0.04%) | 10493562 (98.51%) |
tof9 | 11078964 (100.00%) | 763 (0.01%) | 41620 (0.38%) | 114 (0.00%) | 182186 (1.64%) | 4761 (0.04%) | 10849520 (97.93%) |
tof12 | 12276878 (100.00%) | 15 (0.00%) | 48113 (0.39%) | 263 (0.00%) | 94668 (0.77%) | 6124 (0.05%) | 12127695 (98.78%) |
tof13 | 11089132 (100.00%) | 108 (0.00%) | 26731 (0.24%) | 88 (0.00%) | 305766 (2.76%) | 3839 (0.03%) | 10752600 (96.97%) |
tof14 | 9954739 (100.00%) | 70 (0.00%) | 24712 (0.25%) | 71 (0.00%) | 245656 (2.47%) | 4004 (0.04%) | 9680226 (97.24%) |