Figure 3

Chip-MS for droplets with S. griseus producing streptomycin. (a) Sketch of the chip-to-MS setup. Droplets were injected into a PDMS chip transitioning into a steel capillary positioned in front of the counter electrode plate. A voltage of U = 1.9 kV was constantly applied to the capillary, leading to electrospray ionization of aqueous droplets arriving at the emitter tip. 1–droplets, 2–spacing oil, 3–PDMS chip, 4–steel capillary, 5–PTFE oil drainage, 6–counter electrode plate, 7–electrospray, 8–MS inlet. (b) Intensity of a selected ion at mass-charge ratio of m/z = 582.5 over time. Individual droplets of 200 pL, pre-incubated for 4 days containing fully grown mycelia of S. griseus producing streptomycin were reinjected into the setup (λ ~ 5). The right graph is a zoomed-in excerpt of the left graph.