Figure 4

Inhibition of reporter cells in droplets after 24 h co-incubation. Depicted are red and green fluorescence intensity for droplets after co-incubation. Every data point corresponds to a droplet. Population markers (green, blue, unlabeled) are indicated by colours. Values for fluorescence intensity were derived from images of stationary droplets (example shown on the right panel). Red lines represent the thresholds defined for red and green fluorescence. Within the quadrants the relative frequency is noted. The distributions of abundance for red and green fluorescence intensity are given on the margins having the same scale as the 2D coordinate plot. (a) Assay with B. subtilis 3610 as reporter and S. hygroscopicus as producer in green labeled droplets (233 droplets analyzed) and S. griseus ST036300 as negative control in unlabeled droplets (247 droplets analyzed) and empty droplets with blue label (345 droplets analyzed) as second negative control. (b) Assay with E. coli ECJW992 as reporter and S. collinus as producer in green labeled droplets (289 droplets analyzed) and S. hygroscopicus as negative control in unlabeled droplets (419 droplets analyzed) and empty droplets with blue label (400 droplets analyzed) as second negative control. Displayed images were taken at 10x magnification. 1–bright-field image, 2–dark-field image, 3–red fluorescence, 4–blue fluorescence, 5–green fluorescence, 6–overlay of dark-field, red, blue and green fluorescence images.