Figure 2 | Scientific Reports

Figure 2

From: Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium

Figure 2

Analysis of CD46 and cleaved CASPASE-3A activity from Der p 2-mediated apoptosis of primary upper airway epithelial cells in the asthmatic patients and control subjects. (A) Primary nasal epithelial cells from the asthmatic patients stimulated with Der p 2 (10 μg/ml) and CD46 expression analyzed at 12, 24 and 48 hours by flow cytometry. Representative profiles are shown. (B) Western blot analysis was performed to detect CD46 and cleaved CASPASE-3A activity from Der p 2-treated epithelial cells for 48 hours from the asthmatic patients and control subjects. Statistical data of 30 paired experiments between asthmatic patients and control subjects for the expressions of CD46 (C) and cleaved CASPASE-3A (D) after Der p 2 stimulation for 48 hours. The Kruskal-Wallis test (among multiple groups) and Wilcoxon signed ranked test (after Der p 2) were used. *p < 0.05 after Der p2 and #p < 0.05 compared to the control group.

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