Figure 7
Transfected HMEX carrying miRNA inhibitors can reverse the glycolysis-driven metabolism programming in HADF. (a) Successful transfection of HMEX with RFP RNA and detection of RFP fluorescence compared to HADF without RFP RNA transfection. (b) GlycoRate Test. HADF cells were cultured with HADF exosomes ( + HADF exo), miRNA negative control (TF-HMEX-neg), HMEX transfected with miR-155 inhibitor (TF-HMEX-155), miR-210 inhibitor (TF-HMEX-210) or miR-155 and miR-210 inhibitor (TF-HMEX-155 + 210) or for 18 h and examined for extracellular acidification rate (mPER). (c) Basal Glycolysis. (d) Compensatory Glycolysis is reversed in HMEX transfected with miR-155 and miR-210 inhibitors when compared to HADF incubated with HMEX (TF-HMEX-neg).
