Figure 3
From: The Ras-related gene ERAS is involved in human and murine breast cancer
Increased proliferation and migration in MCF10A cells expressing ERAS. (a) Representative growth curves of MCF10A control and MCF10A-ERAS cells. Proliferation was measured using XTT proliferation assay. Data represent the means ± SEM of three independent experiments. (b) Analysis of the phase distribution of the cell cycle in cells growing at high confluence (85–90%) by flow cytometry in three independent experiments. Note the difference in the scale between the upper and lower parts of the graph. (c) Representative images of an in vitro wound-healing assay at 0, 24 and 48 hours post-wounding. (d) Quantification of the migration area after wounding. Four wounds were analyzed for each cell type in three independent experiments. Migration areas were measured with ImageJ software. In b and d, data represent the means ± SD. Asterisks show significant differences (*p < 0.05; **p < 0.01 and ****p < 0.0001).
