Figure 7
From: The Ras-related gene ERAS is involved in human and murine breast cancer
ERAS promotes the development of larger and more undifferentiated tumors when expressed in breast cancer cells. (a) Growth curves of tumors generated from MDA-MB-231 control and MDA-MB-231-ERAS cells in xenograft assays in nude mice. Tumors (n = 12 for each genotype) were measured on days 15, 19, 23 and 27 after injection. Tumors generated from ERAS-expressing cells grew faster than those arisen from control cells. (b) Weight of tumors extracted on day 27 after injection. (c) Histological characterization of xenograft tumors. Hematoxylin and eosin staining, and HA-tag and keratin K8 immunostaining are shown. Tumors-expressing ERAS did not show any foci of acinar differentiation expressing keratin K8. (d) Activation of AKT pathway in tumors generated from MDA-MB-231 analyzed by western blot. Uncropped blots are presented in Supplementary Fig. S6. (e) Histological images of lung metastasis of different mice injected with MDA-MB-231-ERAS cells. Hematoxylin and eosin staining and HA-tag immunostaining are shown. Asterisks show significant differences (***p < 0.001 and ****p < 0.0001). Bar, 100 μm. Data represent the means ± SEM.
