Figure 4 | Scientific Reports

Figure 4

From: FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway

Figure 4

FR58P1a induces mild uncoupling of OXPHOS without effect on plasma membrane potential. (a) Effect of FR58P1a on flux-force relationship of bioenergetics parameters in MDA-MB-231 cells, cellular respiration (green square), mitosox fluorescence (blue square), intracellular ATP levels (red square), (b) FR58P1a and FCCP produce fragmentation of mitochondrial network of TNBC BT549 and non-tumoral MCF10A cells at 4 h of exposition. (c,d) FR58P1a produces mild uncoupling of OXPHOS, increasing the TCA activity-dependent ECAR value in MDA-MB-231 cells. (e,f) FR58P1a induces a mild mitochondrial NADH oxidation in TNBC cells. (g) Whole cell voltage clamp recording from MDA-MB-231 cells exposure to 30 µM FR58P1a and (h) 1 µM FCCP. Data shown are the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, vs. Control (DMSO).

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