Figure 5 | Scientific Reports

Figure 5

From: FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway

Figure 5

FR58P1a-induced mitochondrial dysfunction activates a cytoprotective Sirt1/AMPK signaling in TNBC cells. (a) Levels of intracellular acetylated-lysine and (b–d) phospho-AMPK levels induced by FR58P1a at 4 h of exposure in TNBC MDA-MB-231 cells, MDA-MB-468 and BT549. (e,f) Effect of Sirt1 inhibition with 10 µM EX-527 (EX) on phospho-AMPK levels induced by FR58P1a in MCF10A and MDA-MB-231 cells, (g,h) FR58P1a does not affect the proliferation and cell cycle progression in TNBC MDA-MB-231 and BT-549 cells, respectively, (i–l) Effect of FR58P1a (30 µM) on MCF10A and TNBC cell death under Sirt1 and AMPK inhibition, with EX-527 and Compound C (CC), respectively at 48 h of exposition. Data shown are the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, vs. Control (DMSO). n.s. not significant.

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