Figure 7 | Scientific Reports

Figure 7

From: FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway

Figure 7

Prolonged FR58P1a treatment triggers metabolic adaptation mediated by mitochondrial clearance, maintaining the inhibitory effect of TNBC cell migration. (a) Changes in the expression of genes involved in the OXPHOS, ADP/ATP transport, mitochondrial biogenesis and glycolysis. The bar chart shows the mean log2(fold change) with p-value ≤ 0.05. In green, genes down-regulated and in red genes that are upregulated. Genes without changes are shown in gray. (b–f) Levels of respiratory complexes. (g,h) Effect of FR58P1a on mitochondrial oxygen consumption rate and 2-NBDG uptake in MDA-MB-231 cells treated by 24 h. (i,j) Effect of FR58P1a on fibronectin-dependent migration in TNBC cells treated by 24 and 72 h. Data shown are the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, vs. Control (DMSO). n.s. not significant.

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