Figure 2
Expression of mGlu5 receptors in cerebellar PCs early after birth. Western blot analysis of mGlu5 receptors in the mouse cerebellum at different PNDs is shown in (A). Densitometric values are means ± S.E.M. of 2–3 mice/group. p = 0.008 vs. values at PND9 (#) or PND12 (*) (One-Way ANOVA + Fisher’s LSD; F(4,9) = 7.01). The β-actin was taken from the same blot but with a lower exposure time. Grm5 mRNA levels and Grm5 promoter methylation in the cerebellum at PND9 and PND18 are shown in (B) and (C), respectively. Values are means ± S.E.M. of 4 mice/group. *p = 0.04 vs. values at PND9 (Student’s t test; t(6) = 2.61), *p = 0.006 vs. values at PND9 (Student’s t test; t(6) = −4.11), for Grm5 mRNA levels and Grm5 promoter methylation, respectively. Confocal microscopy images of mGlu5 receptor immunoreactivity in PCs at different PNDs are shown in D. Images were obtained from two different experiments performed in different laboratories. In the upper panel mGlu5 receptors are in green and type-8 carbonic anhydrase (a marker of PCs) is in red. In the lower panel, mGlu5 receptors are in green and calbindin (another marker of PCs) is in red. Note the disappearance of mGlu5 receptors in PCs after PND12. * indicates PCs; arrowheads indicate mGlu5 receptor immunoreactivity in PCs.
