Figure 1

Representative images of multiplexed fluorescently-labelled NEC sections. Clear and high throughput information was used to define the immune profile. CD3+/CD4 TILs were stained purple (arrow) and CD3+/CD8 TILs were stained cyan (arrow head) (A), and CD3+/PD-1high T cells were stained cyan (arrow) and CD204+/PD-L1high macrophages were stained orange (arrow head) (B).