Figure 2
From: Elucidating the transactivation domain of the pleiotropic transcription factor Myrf
The NTM domain is sufficient for transcriptional activation. (A) NTM-G4 is a fusion between the NTM domain and Gal4 N-terminal fragment (G4). NTM-ΔN30-G4 is the same as NTM-G4 except that the N-terminal 30 residues of the NTM domain are deleted. NTM-N50-G4 is a fusion between the first 50 N-terminal residues of the NTM domain and G4. The transcriptional activity of G4, NTM-G4, NTM-ΔN30-G4, and NTM-N50-G4 was determined by luciferase assay in Oli-neu and HEK293FT cells in panels B and C, respectively. Shown are means and standard errors. *p < 0.01 by two-tailed unpaired Student’s t test with Bonferroni correction. (D) The luciferase assay samples of panels B and C were analysed by Western blot to examine protein expression levels. The grouping of blots cropped from different parts of the same gel is indicated by dividing lines. The full blots are shown in Supplementary Figure 2.
