Figure 7
From: Gene expression profiling during hibernation in the European hamster
Typical example of digital droplet PCR (ddPCR) mRNA quantification. Using ddPCR, the exact number of Per2 mRNA copies was quantified in hypothalamus tissue samples obtained from European hamsters at three different stages of the hibernation cycle (n = 8 from each stage): normothermia, torpor, and arousal. The graph on the left side depicts the calculated concentration of Per2 mRNA copies/µL for each animal in each hibernation stage. The graph on the right side shows the fluorescence intensity of droplets in terms of the droplet number for each sample. QuantaSoft software was used to calculate the numbers of droplets containing Per2 mRNA (blue) or not containing Per2 mRNA (grey) in each sample.
