Table 5 Primer/probe sequences, RNA quantity, and annealing temperatures for digital droplet PCR (ddPCR).

From: Gene expression profiling during hibernation in the European hamster

Genes	Probes^a	Probe References^b	Forward Primer Sequences^c	Primer Reverse Sequences^d	RNA Quantity (ng)^e	Annealing temperature (°C)^f
Per1	UPL 63	4688627001	CCAGCACAACAAAGCGTAAA	TCAGAGGCTGAGGAAGCAGT	1	57
Bmal1	UPL 56	4688538001	CCAACCTTCCCACAGCTTAC	CCTGGAATGCCTGGAACA	5	57
Rev-Erbα	UPL 150	4694368001	TGTCTGTCAGTGGGAATGTCA	CTCTGTTTCTCACGCTTAGGAAT	1	57
Clock	AATGAAGTTACACTCTCAGATACAT	NA	CCACAAGATCAGATGGTA	TAGCGATCATGACAGATG	50	51
Per2	UPL 161	4694481001	CTTCTTGTCTGCAGGGAGGT	TGTCCTTATCAGTTCTTTGTGTGC	10	55
MT ₁	UPL 145	4694317001	CCCTCTGCTACGTGTTCCTG	GAGTTCCGGTTTGCAGATTG	150	59
MT ₂	UPL 131	4694155001	TGTGGTGAGTCTGGTCTTGG	AGGATCAGTGGGTAAGGGTACA	100	57
GPR50	UPL 86	4689119001	GCTGGCTCTTCCTCTAAGCA	GGCTGGTAGCAGGCTTAATG	150	55
QR2	UPL 68	4688678001	AAGACAGCTCTGACCAGTGACA	CTAGATCAGCTTCTTGCACCTTC	5	59
Tubulin	UPL 78	4689011001	GAGCGGCTCTCTGTCGATTA	GGGGCTGGGTAGATGGAG	0.01	59
G6PD	UPL 30	4687639001	TGTGGCAAAGCCCTGAAT	TGCCACATCTCGGAACTGTA	5	55
Actin	UPL 9	4685075001	GCTATGAGCTGCCTGATGG	GGCTGGAAAAGAGCCTCA	0.1	57
SIRT1	UPL 68	4688678001	GAAAGTGCTGGCCCAATAGA	GATTACCATCAAGCCGCTTACTA	5	57
TXNIP	UPL 125	4693604001	CCTTGCTGATCTATGTTAGTGTCC	TCACCAGGGGAAGGTCAA	1	57
PPARα	UPL 56	4688538001	CGGTGTGTATGAAGCCATATTC	ATCAGCATCCCGTCTTTGTT	1	57
PGC1α	UPL 41	4688007001	GTAGGCCCAGGTATGACAGC	CCTTTCAGATTCCCGTTTCTC	1	57
FGF21	ACACTGAAGTCCACCTGG	NA	ACCTCTACACAGATGACA	GGTTGTTGGCAAAGAAC	100	58
Ob-R	UPL 113	4693477001	CGCAGGAGATCAGACCAATC	ATTGATGGCCAGAACCGTAA	100	57
Dio2	UPL 22	4686969001	CCACCTTTCACTAGGCAACTG	AGTCGGCCACTGATGAGAAC	10	57
Dio3	UPL 135	4694198001	GCACCTAACTCGGAGGTCAT	ATAGTCGAGGATGCGCTGTC	100	57
RBM3	UPL 65	4688643001	TGGAAGCGGAAGATATGACA	TCTCTGGACCGCCCATATC	1	57
UCP1	UPL 21	4686942001	GGCAACCTACTGAGGTCGTG	ATCGGGGTTTGATCCCATA	0.1	57

Each ddPCR reaction requires probes^a,b, forward^c and reverse^d primers, and determination of the optimal RNA quantity^e and annealing temperature^f were determined.

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Table 5 Primer/probe sequences, RNA quantity, and annealing temperatures for digital droplet PCR (ddPCR).

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