Figure 1

TSAd impact on proliferation and phenotype of CD4+ T cells. (A) TSAd is expressed in proliferating human CD4+ T cells upon anti-CD3/CD28 stimulation. Dot plots show TSAd expression in relation to CTV dilution, while histogram shows degree of CTV dilution over 4 days with or without stimulation. (B) Dot plots show expression of CD62L and CD44 in Sh2d2a+/+ and Sh2d2a−/− mouse CD4+ T cells from C57/BL6 mice upon anti-CD3/CD28 and IL2 stimulation, over 9 days in vitro. (C) Accumulated proliferation of CD4+ T cells from Sh2d2a+/+ and Sh2d2a−/− mice stimulated as in (B). (D) Relative proportion of Tcm and Tem cells in T-cell cultures stimulated as in (B). Data in C and D represent mean +/−SD of three independent experiments. Statistical significance was assessed by two-way ANOVA. E) Accumulated proliferation of Id-specific TCR transgenic CD4+ T cells from Sh2d2a+/+ and Sh2d2a−/− mice upon culture under Th1 skewing conditions (peptide loaded APC in presence of IL12 and anti-IL4). Data represent mean +/− SD of three independent experiments. Statistical significance was assessed as in C and D. (F) Graph shows the frequency of GB113+ T cells with Tcm or Tem phenotype. Data in G and H represents mean +/− SD of 3 independent experiments, analysed using paired student’s t-test.