Figure 4
From: Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd
Sh2d2a influences the kinetics of CD4+ T cell polarization towards the APC upon TCR-engagement. (A,C,E,G and I) Line charts show the frequency of (B) polarized F-actin (IS-positive conjugates), (C) Id-specific TCR (maturing IS-positive conjugates), (D) PKCξ, G) PAR3 and I) γ-tubulin (within maturing IS-positive conjugates) in Id-specific TCR T:APC conjugates displaying polarization in T cells towards the APC. (B,D,F,H and J) Line charts show the corresponding median polarization ratios towards the IS of Id-specific TCR T cells displaying polarization of F-actin, Id-specific TCR, PKCξ, PAR3 or γ-tubulin respectively. Data represents the median +− range of three separate experiments, n > 50 gated events in each experiment. Significance was determined by two-way ANOVA and Sidak’s multiple comparison test.
