Figure 5
From: Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd
Sh2d2a affects IFNγ in Id-specific TCR CD4+ T cells. (A) Sample images show absence (−) and presence (+) of IFNγ in TCR-Id CD4+ T cells conjugated to Id-positive APC, and displaying polarized F-actin and Id-specific TCR. Images showing expression of IFNγ accumulated to the T cell synapse. (B) Histograms show IFNγ staining of T cells with F-actin and TCR polarisation towards the synapse when conjugated to APC for 30 and 720 minutes respectively. (C) Line charts showing kinetics of IFNγ polarisation to synapse in T cell conjugates upon Id presentation. Data represents the average of 3 separate experiments, n > 50 gated events in each experiment. Significance was determined by two-way ANOVA and Sidak’s multiple comparison test. Mean +/− SD.
