Figure 5
HGF co-treatment prevents dedifferentiation of polarized CFBE cells after prolonged exposure to VX-809. Polarized CFBE-Phe508del cells were treated for 15 days with DMSO, HGF (50 ng/ml), VX-809 (3 μM), or VX-770 (0.5 μM), alone or in the indicated combinations. (a) WB analysis of whole cell lysates. Shown are representative images of immunoblots for ZO-1, E-cadherin, CK8, and CK18 together with bar plots of band intensity quantification, normalized to DMSO (means ± SEM) from three independent assays. Histone H2B was used as a loading normalizer in band intensity quantification. (b–d) Immunofluorescence staining of polarized CFBE-Phe508del cells, treated as in (a), were stained with phalloidin-TRITC, DAPI, and either anti-ZO-1/Alexa 488 (b), anti-CK8/Alexa 488 (c), or anti-CK18/Alexa 488 (d), and analysed by confocal microscopy. Shown are overlay images representative of the indicated treatment conditions. White horizontal bars represent 10 μm. *p < 0.05.
