Figure 7
Prolonged HGF and VX-770 treatments have opposite effects on Ki-67 levels in polarized Caco-2 cells. Polarized Caco-2 colorectal cells were treated for 15 days with DMSO, HGF (50 ng/ml), VX-809 (3 μM), or VX-770 (0.5 μM), alone or in the indicated combinations. Shown are representative WB images of whole cell lysates probed with anti-Ki-67 and anti-Tubulin antibodies. Bar plot shows quantification of Ki-67 band intensity, normalized to DMSO (means ± SEM) from three independent assays. Tubulin was used as a loading normalizer in band intensity quantification.
