Figure 1

Arsenic induces miR-301a expression during cellular transformation. (A) BEAS-2B cells were exposed to arsenic (0.25 μM) for 6 months and the morphology of normal BEAS-2B (BEAS-2B) and transformed BEAS-2B (BEAS-2B-As) cells are present. (B) The expression of miR-301a was determined by qPCR between none transformed BEAS-2B (BEAS-2B) and transformed BEAS-2B (BEAS-2B-As) cells. (C) BEAS-2B cells were exposed to different concentrations of arsenic (0, 2.5, 5, 10 μM) for 12 h and the expression of miR-301a was determined by qPCR. (D) BEAS-2B cells were exposed to 0.25 μM arsenite for 0, 10, 20, 30 passages and the expression of miR-301a was determined by qPCR. Values represented the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 indicate a significant difference between none transformed and transformed cells, or between none treatment and treatment groups.