Figure 4

SMAD4 is a direct target of miR-301a in arsenic transformed BEAS-2B cells. (A) Western blot analyses of miR-301a targets among none transformed BEAS-2B (BEAS-2B) with anti-miR-control, transformed BEAS-2B (BEAS-2B-As) with Anti-miR-control, and transformed BEAS-2B (BEAS-2B-As) with Anti-miR-301a. (B) Prediction of major interference sites between miR-301a and SMAD4 mRNA 3′UTR by using Targetscan. (C) Luciferase activity in BEAS-2B-As cells transfected with the indicated luciferase reporter with either an miR-301a mimic or control oligonucleotide. (D) Western blot analysis of SMAD4 expression in BEAS-2B-As cells with the indicated luciferase reporter with either an miR-301a mimic or control oligonucleotide. Gel pictures were acquired by using Tanon5200 and the exposure time was 30 sec for SMAD4 and 5 sec for β-actin. Values represented the mean ± S.D. *P < 0.05 and **P < 0.01 indicate a significant difference between the indicated groups.