Figure 4

Scatter plot of minimum inhibitory concentrations (MIC) obtained with the MIC-gradient strip test with MBT-ASTRA relative growth (RG) values of 100 recent bovine clinical strains of P. multocida. MBT-ASTRA testing conditions were 3 hours of incubation with a concentration of tetracycline of 4 µg/mL. The horizontal and vertical line represent the clinical breakpoint of ≤2 µg/mL and the RG cut-off value of 0.5, respectively. Two isolates with an MIC value of 6 µg/mL are considered susceptible with MBT-ASTRA, causing 2 false susceptible results.