Figure 5 | Scientific Reports

Figure 5

From: Evolution shapes the responsiveness of the D-box enhancer element to light and reactive oxygen species in vertebrates

Figure 5

D-box regulation via MAP kinase signalling. (A,B) Representative real time luciferase assays of PAC-2 cells transfected with the D-boxcry1a-Luc reporter together with expression vectors for DN-JNK1, DN-p38 or EGFP (negative control) upon exposure to (A) blue LD cycles or to (B) H2O2. (C) Representative real time luciferase assays of PAC-2 cells transfected with the D-boxcry1a-Luc reporter and incubated with different concentrations of the U0126 inhibitor 1 hour before the H2O2 treatment. Mean of bioluminescence (CPS) (n = 8) are plotted on the y-axes and time is plotted on the x-axes. Blue and black bars below the x-axes denote the extent of the blue light and dark periods, respectively.

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