Figure 6
ROS and D-box regulation in HeLa cells (A) DCF assay of HeLa cells exposed during 4 hours to monochromatic blue light. Means of fold induction (n = 24) ± SD with respect to time 0 are plotted on the y-axis, while the duration of light exposure is plotted on the x-axis. (B,C) Representative real time bioluminescence assays in (B) HeLa and (C) PAC-2 cells (control) transfected with the D box-driven-luciferase reporter D-boxcry1a-Luc, treated with H2O2 and after 24 hours exposed to one blue light/dark cycle (D-box Luc + H2O2, red traces). Black traces indicate cells treated with only the vehicle and after 24 hours exposed to one blue light/dark cycle (D-box Luc). Means of relative bioluminescence (n = 8) are plotted on the y-axis and time on the x-axis. Blue and black bars below the graphics indicate the different lighting regimes during the experiments. See also Fig. S2 for controls.
