Figure 5
From: MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network
MARCKS interacts with CDC42, but does not influence whole brain levels of CDC42 activation. (a–c) CDC42 (green, a) colocalizes with MARCKS (red, b) in developing neurites (white arrowhead, c). (d) CDC42 coimmunoprecipitates with MARCKS from E18.5 mouse whole-brain lysates. (e–g) Levels of GTP-bound (active) CDC42 (PAK1 PD) are not significantly different in E18.5 brain lysates from Marcks+/+, Marcks−/+ and Marcks−/− embryos. Lysates (input, separate gel) blotted for total CDC42 and GAPDH serve as loading controls. (f,g) Quantification of CDC42 activity assay shown in (e). One-way ANOVA, *p < 0.05 level using post-hoc Tukey’s correction for multiple comparisons. n = 3/genotype. Bar graphs are mean ± SEM. Scale bar in (c) is 10 μm.
