Figure 3
Glycerol release and the effect of adipocyte volume during inhibition of HSL activity. Least squares means of basal glycerol release (nmol/106 adipocytes per 3 h) (A), CAY inhibited basal glycerol release (% decrease of basal glycerol release) (B), isoproterenol (ISO) stimulated glycerol release (% increase of basal glycerol release) (C), CAY inhibited ISO stimulated glycerol release (% decrease of ISO stimulated glycerol release) (D) and insulin (0.2 and 1 µg/L) inhibited ISO stimulated glycerol release (% decrease of ISO stimulated glycerol release) (E,F). Effect of adipocyte volume on basal glycerol release (G) and CAY inhibited ISO stimulated glycerol release (H) in SCAT explants from Holstein dairy cows at dry (−11 ± 1 d), fresh (+11 ± 0.2 d) and lactation (+24 ± 0.4 d relative to calving). Error bars represent the SEM. Time-points with different letters differ significantly (ab, P < 0.05). P-values for the effect of period and adipocyte size (volume in pL) on the different variables are derived from the linear mixed effect model.
