Figure 2

N-linked glycosylation deficiency enhances PrP toxicity in SH-SY5Y cells induced by the toxic prion peptide PrP 106–126. SH-SY5Y cells transiently expressing wild-type PrP (a), V180I (b), N197D (c), or the double mutant N181D/N197D (d) were cultured for 2 days and incubated with 60 μM PrP 106–126 for 2 days. The percentage of apoptotic cells was determined by flow cytometry as described in the legend of Fig. 7.