Figure 2
BET inhibitors decrease IFN-γ-induced PD-L1 expression in PDAC cells and PSCs. CD18 PDAC cells, primary PSC #1, and the pancreatic stellate cell line were pre-treated with the BET inhibitors JQ1 (1 μM) or I-BET151 (I-BET; 1 μM) for 30 minutes and treated with IFN-γ (0.2 μg/mL) for 24 hours. (A–C) The effect on PD-L1 mRNA expression was determined by qRT-PCR. (D–F) The effect on PD-L1 protein expression was determined by Western blotting and by FACS analysis. PD-L1 and HSP90 bands from three independent experiments were quantified by densitometry and data expressed as relative protein ratios. The gene expression results are representative of five independent experiments. Bar graphs represent means +/−S.D. *p < 0.05; **p < 0.01; ***p < 0.001 relative to control samples.
