Figure 3
BET PROTAC ARV-825 decreases IFN-γ-induced PD-L1 expression in PSCs. Primary PSC #1 and the pancreatic stellate cell line were pre-treated with the BET PROTAC ARV-825 (0.25 μM) for 30 minutes and treated with IFN-γ (0.2 μg/mL) for 24 hours. (A,B) The effect on BRD2, BRD3, BRD4 and PD-L1 protein expression was determined by Western blotting. PD-L1 and HSP90 protein bands from three independent experiments were quantified by densitometry and data expressed as relative protein ratios. (C,D) The effect on PD-L1 mRNA expression was determined by qRT-PCR. The gene expression results are representative of three independent experiments. Bar graphs represent means +/−S.D. ns, not significant; *p < 0.05; **p < 0.01 relative to control samples.
