Figure 4
BRD4 knockdown decreases IFN-γ-induced PD-L1 expression in PSCs. (A,B) Primary PSC #1 and the pancreatic stellate cell line were transfected with control siRNA or with siRNAs against BRD2, BRD3 or BRD4 for 48 hours. The cells were then treated with IFN-γ (0.2 μg/mL) for 24 hours. (A,B) The effect on the individual BRD proteins was determined by Western blotting. Bands from three independent experiments were quantified by densitometry and data expressed as relative ratios. (C,D) The effect on the individual BRD mRNAs was determined by qRT-PCR. (E,F) The effect on PD-L1 mRNAs was determined by qRT-PCR. The gene expression results are representative of four independent experiments. Bar graphs represent means +/− S.D. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 relative to control samples.
