Figure 5
c-MYC knockdown does not decrease IFN-γ-induced PD-L1 expression. (A,B) Primary PSCs and the pancreatic stellate cell line were transfected with control siRNA or with siRNA against c-MYC for 48 hours. The cells were then treated with IFN-γ (0.2 μg/mL) for 24 hours. The effect on c-MYC was determined by qRT-PCR and by Western blotting, and the effect on PD-L1 mRNA was determined by qRT-PCR. c-MYC and GAPDH bands from three independent experiments were quantified by densitometry and data expressed as relative ratios. The gene expression results are representative of three independent experiments. Bar graphs represent means +/− S.D. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 relative to control samples.
