Figure 7
BET inhibitors do not suppress IFN-γ-induced IRF1 protein expression. (A) Primary PSCs and the pancreatic stellate cell line were pre-treated with the BET inhibitors JQ1 (1 μM) or I-BET151 (I-BET; 1 μM) for 30 minutes and then treated with IFN-γ (0.2 μg/mL) for 4 hours. The effect on IRF1 mRNA and protein expression was determined by qRT-PCR and by Western blotting. (B) Primary PSCs and the pancreatic stellate cell line were transfected with control siRNA or with siRNA against BRD4 for 48 hours. The cells were then treated with IFN-γ (0.2 μg/mL) for 4 hours. The effect on BRD4 protein was determined by qRT-PCR and by Western blotting, and the effect on IRF1 mRNA was determined by qRT-PCR. Bands from three independent experiments were quantified by densitometry and data expressed as relative ratios. The gene expression results are representative of three independent experiments. Bar graphs represent means +/− S.D. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 relative to control samples.
