Figure 4

Immunohistochemical characteristics of the TiHo-0906 cell line. (a) E-cad, low passage (P8); most cells display a weak cytoplasmic and membranous labelling. (b) E-cad, high passage (P80); weak cytoplasmic and membranous labelling in most cells, some cells display a more intense reaction. (c) pan-CK, low passage (P8); moderate cytoplasmic labelling, some cells display a more intense reaction. (d) pan-CK, high passage (P80); moderate cytoplasmic labeling. (e) Vim, low passage (P8); strong cytoplasmic labelling and numerous positive cells. (f) Vim, high passage (P80); most of the cells are moderately positive, some cells are strongly positive. (g) p63, low passage (P8); moderate nuclear labelling in most of the cells, some nuclei are strongly positive. (h) CD44, low passage (P8); cellular membranes are moderately positive. (i) CD44, high passage (P80); moderate membranous labelling in most of the cells, some cells are strongly positive.