Figure 9

Flow cytometric assessment of doxorubicin effects on TiHo-0906 cells. (a) Percentage of intact cells (1^st and 2^nd column, low and high passage; respectively), and cell debris (3^rd and 4^th column, low and high passage; respectively) after incubation with doxorubicin. Cellular debris increases in proportion to the concentration of doxorubicin while intact cells decrease. Notice the higher amount of intact cells compared with cell debris at all doxorubicin concentrations tested. (b) In both passages tested viable cells decrease in a dose-dependent manner. Cells at low passage were more resistant to higher concentrations of doxorubicin (significance bars), except for 1000 nM. (c) The amount of apoptotic cells rises in parallel to the concentration of doxorubicin. Doxorubicin-induced Apoptosis was higher in cells at high passages in almost all concentrations tested (significance bars). (d) Incubation with doxorubicin was not able to give a significant increase of dead cells. Data are displayed as mean (SD); *p < 0.05, **p < 0.01, and ***p < 0.001.