Figure 1

Sequence analysis of BoNAC019, nuclear localization and transcriptional activation of BoNAC019. (A) Phylogenetic tree of BoNAC019 and NAC members from other plant species. (B) Subcellular localization of the BoNAC019 protein in onion epidermal cells. (C) Transcription activation activity of BoNAC019. The full-length proteins (BoNAC019-FL), N-terminal fragment (BoNAC019-N) and C-terminal fragment (BoNAC019-C) were fused with the vector GAL4BD. (D) The plasmids containing the fusion genes and the empty control plasmid pGBKT7 were introduced into protoplasts. The GAL4BD vector was used as a negative control.