Figure 4
Fiber blocking assays in various cell lines. Adherent Hela cells were pre-incubated with increasing concentrations of recombinant fiber knob proteins (HAdV5 knob, HAdV3 knob, HAdV35 knob, HAdV17 knob or a combination) and then incubated with (A) HAdV3GFP, HAdV5/35GFP, and HAdV5GFP, and (B) HAdV17GFP. Uninfected cells (negative controls) were used to set the background gate at approximately 1%. MFI indicates mean fluorescence intensity. (C) HeLa cells were pretreated with HAdV35 knob, HAdV5 knob and HAdV17 knob at increasing concentrations at 37 °C for 1 hour, and then exposed to HAd17GFP at MOI 100. GFP expression levels (MFI) were measured 24 hrs later by flow cytometry. (D) EA.hy926 cells and (E) CHO-C2 were pretreated with HAdV35 knob at increasing concentrations at 37 for 1 hour, and then exposed to HAdV17GFP and HAdV35GFP virus at MOI 100. GFP expression (MFI) was measured 24 hrs post-infection by flow cytometry.
