Figure 5
Gain of function study to validate endothelium tropism that is dependent on interaction between CD46 and HAdV17 fiber. (A) Schematic representation of chimeric fiber proteins incorporated into HAdV5 capsid and structure of chimeric fiber genes (knob, shaft and tail). The white fiber is derived from HAdV5GFP and the black fiber from HAdV17GFP. HAdV5GFP/17knob contained the shaft and tail from HAdV5 and knob from HAdV17, whereas HAdV5GFP/17fiber contained the tail from HAdV5 and both shaft and knob from HAdV17. (B) HEK293- and EA.hy926 cells were transduced with HAdV5GFP, HAdV5GFP/17fiber, HAdV5GFP/17Knob and HAdV17GFP at various multiplicities of infection (0.1, 1, 10 or 100). GFP expression levels were analyzed 24 h post-infection by FACS. Uninfected cells (negative controls) were used to set the background gate at approximately 1%. Percentages provided indicate number of GFP-positive cells (left panel). MFI represented mean fluorescent intensity (right panel). Data points represent mean standard error out of 3 independent experiments (n = 3). ***P < 0.001.
